optimization of sample preparation for 1-hydroxypyrene as a major biomarker of exposure to polycyclic aromatic hydrocarbons prior to high performance liquid chromatography
نویسندگان
چکیده
urinary 1-hydroxypuren (1-ohp) is commonly used as a major metabolite and biological indicator of the overall exposure to polycyclic aromatic hydrocarbons (pahs). for evaluation of human exposure to such compounds, biological monitoring is an essential process, in which, preparation of samples is one of the most time-consuming and error-prone aspects prior to chromatographic techniques. in this study, non classic form of liquid-liquid extraction (lle) was optimized with regard to solvent type, solvent volume, extraction temperature, mixing type, and mixing duration. through the extraction process, a mild temperature was used to keep the compound of interest as stable as possible. in this study, a high performance liquid chromatography, using reverse-phase column was used. the isocratic run was done at a constant flow rate of 0.8 ml/min, the mobile phase was methanol/water and a fluorescence detector was used, setting at 242 nm and 388 nm. at the developed conditions, the extraction recovery was exceeded 87.3%, achieving detection limit of 0.2 µg/l. the factors were evaluated statically and the procedure was validated with three different pools of spiked urine samples and showed a good reproducibility over six consecutive days as well as experiments. it was concluded that, this optimized method could simplify sample preparation for trace residue analysis of pahs metabolites.
منابع مشابه
Solid Phase Extraction for 1-Hydroxypyrene as a Biomarker of Occupational Exposure to PAHs Prior to High Performance Liquid Chromatography
Urinary 1-hydroxypyrene is frequently used as a major metabolite and biological indicator of the overall exposure to polycyclic aromatic hydrocarbons. In this study, solid phase extraction was appropriately conditioned with regard to sample pH, sample concentration, loading flow rate, elution solvent, washing solvent, sample volume and sorbent mass. Octadecyl silica cartridge (C<su...
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urinary 1-hydroxypyrene is frequently used as a major metabolite and biological indicator of the overall exposure to polycyclic aromatic hydrocarbons. in this study, solid phase extraction was appropriately conditioned with regard to sample ph, sample concentration, loading flow rate, elution solvent, washing solvent, sample volume and sorbent mass. octadecyl silica cartridge (c18) was used as ...
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In order to capture the extent of exposure to polycyclic aromatic hydrocarbons (PAHs), various biomarkers have been employed. The biomarkers employed for PAHs include PAHs genetoxic end points in lymphocytes, urinary metabolites, PAH-DNA adducts, and PAH-Protein adducts. Of these, excretory 1-hydroxypyene, a metabolite of pyrene, has been used extensively as a biological monitoring indicator of...
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عنوان ژورنال:
iranian journal of public healthجلد ۳۵، شماره ۱، صفحات ۳۳-۴۱
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